Analysis Note

ControlU266 cells

Application

Research Sub CategoryImmunoglobulins & Immunology

Detect CD138-APC using this Milli-Mark Anti-CD138-APC Antibody, clone MI15 validated for use in FC.

Research CategoryInflammation & Immunology

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

CD138 is a transmembrane proteoglycan, bearing heparin sulphates and sometimes chondriotin sulfates. It consists of a 30.5 kDa core protein with 5 putative glycosaminoglycans attachment sites. CD138 is a member of the syndecan family. The reported Mr of CD138 ranges from 85,000 to 92,000, presumably depending on the glycosaminoglycan pattern of the molecule (Wijdenes, 1997). Within the hematopoietic system, CD138 is mainly confined to late stages of B-cell differentiation. It is typically expressed at high cellular density in plasma cells (Gattei, 1999). In mature tissues, CD138 is expressed by simple and stratified epithelia, fibroblasts, stratified keratinocytes and endothelial cells (Wijdenes, 1997). Anti-CD138, MI15, was included in the Sixth International Workshop and Conference on Human Leucocytes Differentiation Antigens, and studies by a number of different laboratories confirmed its reactivity with CD138 (Wijdenes, 1997).

Immunogen

A mixture of U266 and XG-1 human myeloma cell lines

Legal Information

MILLI-MARK is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Protein A purified

Purified Mouse monoclonal IgG1 conjugated to APC in 0.05M Tris-HCl buffer with 1% BSA, Sodium Azide at 0.097% at pH 7.2.

Quality

Evaluated by flow cytometry using U266 cells

Specificity

Antibody recognizes Human CD138 antigen.

Storage and Stability

Maintain refrigerated at 2-8°C protected from light in undiluted aliquots for up to 6 months from date of receipt.

Target description

85-92 kDa (Wijdenes, 1997)